-
Utilizes
interchangeable professional fluorescence cubes to create images
compatible with most existing camera systems.
- Much lower cost and smaller (more compact) than a
traditional microscope.
- Field of view up to 9.2 mm with a 2/3” CCD in macro mode.
- Micro mode utilizes professional infinity corrected
objectives.
- Remote UV light source includes a long-life metal halide
bulb.
- Zoom and fixed systems available. Fine focus
included with both systems.
Navitar’s Video ZFL Scope is a Macro/Micro fluorescent
vision system that utilizes interchangeable professional fluorescent cubes
and internal focus to create an image compatible with most existing camera
systems. It is a simple means of doing very sophisticated, task oriented
fluorescence without the expense and complexity associated with a fully
loaded research microscope.
Basic Components
of Any Video Fluorescent System
-
A
light source emitting the wavelengths required to cause
the labeling dye to fluoresce. Two different remote light sources are
available, a halogen light for the longer wavelengths, and a metal arc
lamp for the UV. LED illumination is currently under development.
- An integrated cube that optimizes
performance by stopping all but the desired (excitation) wavelength from
reaching the object and then stopping all but the fluorescing wavelength
(emitting) from reaching the camera. There is a multitude of off-the-shelf
cubes available depending on which labeling dye is being used.
The Navitar system permits the usage of all standard Olympus BX2
Fluorescent Cubes, which are available from multiple sources. These are
captured singularly in a quick change holder requiring only a minute to
interchange. Information on filter sets and cubes can be found at
www.chroma.com.
- A camera whose sensitivity and bandwidth
are adequate to handle the fluorescing light levels (which can sometimes
be minimal).
All other components are optics and mechanics that permit
variation of the magnification (field of view) and resolution, along with a
means of achieving critical focus.
Navitar System is Suitable for
Operation in Macro or Micro Mode
Macro Mode
In the Macro mode, a fixed lens system provides up to a 9.2 mm diameter
field of view at a 165 mm working distance with a 2/3" format camera. A 6.5X
zoom accessory provides up to a 15 mm diameter field at a 108 mm working
distance. Various lens attachments allow the working distance to change from
32 mm to 165 mm. An add-on focus accessory provides a means of adjusting
focus without requiring a microscope type stand.
Micro Mode
In the Micro mode, the same single lens system provides a 0.9X coupling of
any standard 200 mm tube length, infinity corrected, fluorescent microscope
objective to the camera.
Cellulose Fiber Images in Macro and
Micro Modes
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Macro Mode
0.5X Lens Attachment
Magnification* = 0.9X
W.D. = 175
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Micro Mode
4X Objective
Magnification* = 3.6X
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Micro Mode
20X Objective
Magnification* =18X
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| *Magnifications are at the image
plane. |
Who is Using
the Navitar Video ZFL Scope?
- OEM customers creating an automated system for scanning
and post processing of proteomics.
- Production laboratory using image processing software to
count and categorize selected images.
- High school or university professor, on a limited budget,
who needs to demonstrate certain fluorescent characteristics to a group.
- A field operator who needs instant results involving
agriculture, oceanography, or criminology.
- Museum or aquarium setting-up a public display.
- Research scientists in government laboratories.
Ideal Uses for the Navitar Video
ZFL Scope
- Transgenic organisms.
- All GFP (Green Fluorescent Protein) studies
involving living cells and tissues: C. Elegans, D. Melanogaster, D.
Rerio Larva, Zebra Fish, Oocytes, Nematodes, Drosophilia.
- Plant cells, plant surfaces, soil samples,
parasites.
- Air and water pollution.
- Forensic - fingerprints, fibers, documents, and
body fluids.
- Capillary flow.
- Bacteria on agricultural products.
- Art restoration.
- Other non-destructive testing.
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- Wafer Contamination by organic residue.
- Inspection of photoresist.
- Diagnosis of lumber diseases.
- Inclusions, imperfections, and compound
variation in geological crystals (including the gem industry).
- Inspection of the structure and chemical
composition of concrete.
- Investigation of the presence and dispersions
of additives or impurities in polymers and ceramics.
- Investigation of structural abnormalities in
materials (cracks, pores, welds).
- Confirming presence of adhesives in cemented
applications.
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